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plko tet on empty vector lentiviral control  (Addgene inc)


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    Structured Review

    Addgene inc plko tet on empty vector lentiviral control
    Plko Tet On Empty Vector Lentiviral Control, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plko+tet+on+empty+vector+lentiviral+control/pLKO-Tet-On-shRNA-Control+(Plasmid+%2398398)/pmc12335957-41-11-16
    Average 93 stars, based on 53 article reviews
    plko tet on empty vector lentiviral control - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Expressing:

    Article Title: GCK inhibition enhances iberdomide antimyeloma effects by promoting IKZF1 degradation via a CRBN-independent mechanism
    Article Snippet: .. To silence GCK expression, human MM cells were transduced with either pLKO-Tet-On empty vector lentiviral control (Addgene) or a pLKO-Tet-On GCK-targeting shRNA lentiviral construct (5′-CAGTTTCACCAGGTGAAATTT-3′), as described before. .. Briefly, protein was extracted from cells using radioimmunoprecipitation assay (RIPA) buffer containing Halt Protease and Phosphatase Inhibitor Cocktail (Thermo Fisher Scientific, Rockford, IL).

    Transduction:

    Article Title: GCK inhibition enhances iberdomide antimyeloma effects by promoting IKZF1 degradation via a CRBN-independent mechanism
    Article Snippet: .. To silence GCK expression, human MM cells were transduced with either pLKO-Tet-On empty vector lentiviral control (Addgene) or a pLKO-Tet-On GCK-targeting shRNA lentiviral construct (5′-CAGTTTCACCAGGTGAAATTT-3′), as described before. .. Briefly, protein was extracted from cells using radioimmunoprecipitation assay (RIPA) buffer containing Halt Protease and Phosphatase Inhibitor Cocktail (Thermo Fisher Scientific, Rockford, IL).

    Plasmid Preparation:

    Article Title: GCK inhibition enhances iberdomide antimyeloma effects by promoting IKZF1 degradation via a CRBN-independent mechanism
    Article Snippet: .. To silence GCK expression, human MM cells were transduced with either pLKO-Tet-On empty vector lentiviral control (Addgene) or a pLKO-Tet-On GCK-targeting shRNA lentiviral construct (5′-CAGTTTCACCAGGTGAAATTT-3′), as described before. .. Briefly, protein was extracted from cells using radioimmunoprecipitation assay (RIPA) buffer containing Halt Protease and Phosphatase Inhibitor Cocktail (Thermo Fisher Scientific, Rockford, IL).

    Control:

    Article Title: GCK inhibition enhances iberdomide antimyeloma effects by promoting IKZF1 degradation via a CRBN-independent mechanism
    Article Snippet: .. To silence GCK expression, human MM cells were transduced with either pLKO-Tet-On empty vector lentiviral control (Addgene) or a pLKO-Tet-On GCK-targeting shRNA lentiviral construct (5′-CAGTTTCACCAGGTGAAATTT-3′), as described before. .. Briefly, protein was extracted from cells using radioimmunoprecipitation assay (RIPA) buffer containing Halt Protease and Phosphatase Inhibitor Cocktail (Thermo Fisher Scientific, Rockford, IL).

    shRNA:

    Article Title: GCK inhibition enhances iberdomide antimyeloma effects by promoting IKZF1 degradation via a CRBN-independent mechanism
    Article Snippet: .. To silence GCK expression, human MM cells were transduced with either pLKO-Tet-On empty vector lentiviral control (Addgene) or a pLKO-Tet-On GCK-targeting shRNA lentiviral construct (5′-CAGTTTCACCAGGTGAAATTT-3′), as described before. .. Briefly, protein was extracted from cells using radioimmunoprecipitation assay (RIPA) buffer containing Halt Protease and Phosphatase Inhibitor Cocktail (Thermo Fisher Scientific, Rockford, IL).

    Construct:

    Article Title: GCK inhibition enhances iberdomide antimyeloma effects by promoting IKZF1 degradation via a CRBN-independent mechanism
    Article Snippet: .. To silence GCK expression, human MM cells were transduced with either pLKO-Tet-On empty vector lentiviral control (Addgene) or a pLKO-Tet-On GCK-targeting shRNA lentiviral construct (5′-CAGTTTCACCAGGTGAAATTT-3′), as described before. .. Briefly, protein was extracted from cells using radioimmunoprecipitation assay (RIPA) buffer containing Halt Protease and Phosphatase Inhibitor Cocktail (Thermo Fisher Scientific, Rockford, IL).



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    93
    Addgene inc plko tet on empty vector lentiviral control
    Plko Tet On Empty Vector Lentiviral Control, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plko+tet+on+empty+vector+lentiviral+control/pLKO-Tet-On-shRNA-Control+(Plasmid+%2398398)/pmc12335957-41-11-16
    Average 93 stars, based on 1 article reviews
    plko tet on empty vector lentiviral control - by Bioz Stars, 2026-10
    93/100 stars
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